bio‐rad mini transblot cell at 90a for 16 h Search Results


bio rad mini protean ii transblot system  (Bio-Rad)
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Bio-Rad bio rad mini protean ii transblot system
Bio Rad Mini Protean Ii Transblot System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nitrocellulose membranes  (Bio-Rad)
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Bio-Rad nitrocellulose membranes
FIG. 3. Identification of vimentin in the cytoskeletal preparation of astrocyte culture by immunoblot analysis. Equal amounts (50 mg) of cytoskeletal proteins were resolved by gel electrophore- sis, transferred to <t>nitrocellulose</t> membrane, and then probed with monoclonal antibody to vimentin, as described in Materials and Methods. A: Age-related alterations in vimentin and its variants in normal (N) and hypothyroid (H) cultures. Note the appearance of the low molecular mass variants of vimentin from day 10 onward. B: Effect of TH (T3 5 0.31 nM and T4 5 22 nM) supplementation for the last 3 days on the expression of vimen- tin in 10-day-old hypothyroid cultures.
Nitrocellulose Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nitrocellulose membrane  (Bio-Rad)
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Bio-Rad nitrocellulose membrane
Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to <t>nitrocellulose</t> filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.
Nitrocellulose Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nitrocellulose membrane - by Bioz Stars, 2026-11
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pvdf membranes  (DuPont de Nemours)
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DuPont de Nemours pvdf membranes
Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to <t>nitrocellulose</t> filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.
Pvdf Membranes, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glass plates  (Bio-Rad)
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Bio-Rad glass plates
Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to <t>nitrocellulose</t> filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.
Glass Plates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds polyacrylamide gel electrophoresis  (Bio-Rad)
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Bio-Rad sds polyacrylamide gel electrophoresis
Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to <t>nitrocellulose</t> filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.
Sds Polyacrylamide Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds polyacrylamide gel electrophoresis - by Bioz Stars, 2026-11
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transblotting chamber  (Bio-Rad)
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Bio-Rad transblotting chamber
Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to <t>nitrocellulose</t> filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.
Transblotting Chamber, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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urea glycerol gel electrophoresis  (Bio-Rad)
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Bio-Rad urea glycerol gel electrophoresis
Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to <t>nitrocellulose</t> filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.
Urea Glycerol Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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avidin horseradish peroxidase conjugate  (Bio-Rad)
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Bio-Rad avidin horseradish peroxidase conjugate
Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to <t>nitrocellulose</t> filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.
Avidin Horseradish Peroxidase Conjugate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sodium dodecyl sulfate polyacrylamide gel electrophoresis  (Bio-Rad)
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Bio-Rad sodium dodecyl sulfate polyacrylamide gel electrophoresis
Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to <t>nitrocellulose</t> filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.
Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyvinylidene difluoride (pvdf) membranes  (DuPont de Nemours)
90
DuPont de Nemours polyvinylidene difluoride (pvdf) membranes
Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to <t>nitrocellulose</t> filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.
Polyvinylidene Difluoride (Pvdf) Membranes, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 3. Identification of vimentin in the cytoskeletal preparation of astrocyte culture by immunoblot analysis. Equal amounts (50 mg) of cytoskeletal proteins were resolved by gel electrophore- sis, transferred to nitrocellulose membrane, and then probed with monoclonal antibody to vimentin, as described in Materials and Methods. A: Age-related alterations in vimentin and its variants in normal (N) and hypothyroid (H) cultures. Note the appearance of the low molecular mass variants of vimentin from day 10 onward. B: Effect of TH (T3 5 0.31 nM and T4 5 22 nM) supplementation for the last 3 days on the expression of vimen- tin in 10-day-old hypothyroid cultures.

Journal: Journal of Neurochemistry

Article Title: Thyroid Hormone-Induced Maturation of Astrocytes Is Associated with the Expression of New Variants of Vimentin and Their Phosphorylation

doi: 10.1046/j.1471-4159.1999.01964.x

Figure Lengend Snippet: FIG. 3. Identification of vimentin in the cytoskeletal preparation of astrocyte culture by immunoblot analysis. Equal amounts (50 mg) of cytoskeletal proteins were resolved by gel electrophore- sis, transferred to nitrocellulose membrane, and then probed with monoclonal antibody to vimentin, as described in Materials and Methods. A: Age-related alterations in vimentin and its variants in normal (N) and hypothyroid (H) cultures. Note the appearance of the low molecular mass variants of vimentin from day 10 onward. B: Effect of TH (T3 5 0.31 nM and T4 5 22 nM) supplementation for the last 3 days on the expression of vimen- tin in 10-day-old hypothyroid cultures.

Article Snippet: Western blotting and immunological detection of vimentin The cytoskeletal proteins analyzed by one-dimensional SDS-PAGE and two-dimensional gel electrophoresis were transferred to nitrocellulose membranes (150 mA, 16 h) using a Bio-Rad transblot apparatus in accordance with the method of Towbin et al. (1979).

Techniques: Western Blot, Membrane, Expressing

Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to nitrocellulose filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.

Journal: The Journal of endocrinology

Article Title: IGFBPs modulate IGF-I- and high glucose-controlled growth of human retinal endothelial cells.

doi: 10.1677/joe.0.1710273

Figure Lengend Snippet: Figure 4 Differential regulation of the four forms of IGFBPs secreted by HRECs. Conditioned media were exposed to 1 nM GH and 10 nM VEGF, insulin, IGF-I, bFGF and PDGF and then electrophoresed through 10% SDS polyacrylamide gels, transferred to nitrocellulose filters and probed with [125I]IGF-II. Known molecular mass standards were run in a parallel lane, as shown on the right. A representative gel is presented. The intensity of the bands was obtained after 3 days of exposure of the autoradiogram.

Article Snippet: Proteins separated on the gel were then transferred onto a nitrocellulose membrane at 10 V for 16 h, using a transblotting cell apparatus (Bio-Rad).

Techniques: